Application of novel environmental (eDNA) techniques for the surveillance of mosquito species from water samples

Thomas Curran, David O'Neill, Allan D. McDevitt, Catherine O'Reilly, Denise O'Meara

Research output: Contribution to conferencePoster


In 1991 an Irish checklist of mosquito species was complied where 18 species were recorded. This is fewer than the checklist for Great Britain (32 species). Therefore concerns with under-recording surrounds this Irish mosquito checklist.

Across Europe cases of mosquito borne diseases (e.g.: malaria, dengue and West Nile fever) have been recorded. These outbreaks have been associated with changes in climate, land use, urbanisation and agricultural practices. The increased mobility of the human population has also furthered the globalisation of these outbreaks which furthers the potential of widespread transmission of vectors and associated pathogens.

Work for this project will involve the collection of mosquito larvae and adults with the aim of developing species specific real-time polymerase chain reaction (qPCR) assays to identify mosquito species from water samples. Additional work will include the application of the method to dietary analysis of mosquito predators, such as bats, and blood meal assessment of adult mosquitoes.

Molecular work will include DNA extraction, conventional and real time PCR, DNA sequencing and primer design. A pilot study involving next generation sequencing and DNA metabarcoding will be completed at the University of Salford.
Original languageEnglish (Ireland)
Publication statusPublished - Mar 2019
EventRoyal Entomological Society Annual Meeting - Co. Down, Newry, Ireland
Duration: 01 Mar 2019 → …


ConferenceRoyal Entomological Society Annual Meeting
Period01/03/2019 → …


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