TY - JOUR
T1 - Circulating angiogenic cell response to sprint interval and continuous exercise
AU - O’Carroll, Louis
AU - Wardrop, Bruce
AU - Murphy, Ronan P.
AU - Ross, Mark D.
AU - Harrison, Michael
N1 - Funding Information:
Acknowledgements This study was supported by Technological Sector Research Strand I funding to Waterford Institute of Technology.
Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/3/6
Y1 - 2019/3/6
N2 - Introduction: Although commonly understood as immune cells, certain T lymphocyte and monocyte subsets have angiogenic potential, contributing to blood vessel growth and repair. These cells are highly exercise responsive and may contribute to the cardiovascular benefits seen with exercise. Purpose: To compare the effects of a single bout of continuous (CONTEX) and sprint interval exercise (SPRINT) on circulating angiogenic cells (CAC) in healthy recreationally active adults. Methods: Twelve participants (aged 29 ± 2 years, BMI 25.5 ± 0.9 kg m − 2 , V˙ O 2 peak 44.3 ± 1.8 ml kg − 1 min − 1 ; mean ± SEM) participated in the study. Participants completed a 45-min bout of CONTEX at 70% peak oxygen uptake and 6 × 20 s sprints on a cycle ergometer, in a counterbalanced design. Blood was sampled pre-, post-, 2 h and 24 h post-exercise for quantification of CAC subsets by whole blood flow cytometric analysis. Angiogenic T lymphocytes (T ANG ) and angiogenic Tie2-expressing monocytes (TEM) were identified by the expression of CD31 and Tie2, respectively. Results: Circulating (cells µL − 1 ) CD3 + CD31 + T ANG increased immediately post-exercise in both trials (p < 0.05), with a significantly greater increase (p < 0.05) following SPRINT (+ 57%) compared to CONTEX (+ 14%). Exercise increased (p < 0.05) the expression of the chemokine receptor CXCR4 on T ANG at 24 h. Tie2-expressing classical (CD14 ++ CD16 − ), intermediate (CD14 ++ CD16 + ) and non-classical (CD14 + CD16 ++ ) monocytes and circulating CD34 + CD45 dim progenitor cells were higher post-exercise in SPRINT, but unchanged in CONTEX. All post-exercise increases in SPRINT were back to pre-exercise levels at 2 h and 24 h. Conclusion: Acute exercise transiently increases circulating T ANG , TEM and progenitor cells with greater increases evident following very high intensity sprint exercise than following prolonged continuous paced endurance exercise.
AB - Introduction: Although commonly understood as immune cells, certain T lymphocyte and monocyte subsets have angiogenic potential, contributing to blood vessel growth and repair. These cells are highly exercise responsive and may contribute to the cardiovascular benefits seen with exercise. Purpose: To compare the effects of a single bout of continuous (CONTEX) and sprint interval exercise (SPRINT) on circulating angiogenic cells (CAC) in healthy recreationally active adults. Methods: Twelve participants (aged 29 ± 2 years, BMI 25.5 ± 0.9 kg m − 2 , V˙ O 2 peak 44.3 ± 1.8 ml kg − 1 min − 1 ; mean ± SEM) participated in the study. Participants completed a 45-min bout of CONTEX at 70% peak oxygen uptake and 6 × 20 s sprints on a cycle ergometer, in a counterbalanced design. Blood was sampled pre-, post-, 2 h and 24 h post-exercise for quantification of CAC subsets by whole blood flow cytometric analysis. Angiogenic T lymphocytes (T ANG ) and angiogenic Tie2-expressing monocytes (TEM) were identified by the expression of CD31 and Tie2, respectively. Results: Circulating (cells µL − 1 ) CD3 + CD31 + T ANG increased immediately post-exercise in both trials (p < 0.05), with a significantly greater increase (p < 0.05) following SPRINT (+ 57%) compared to CONTEX (+ 14%). Exercise increased (p < 0.05) the expression of the chemokine receptor CXCR4 on T ANG at 24 h. Tie2-expressing classical (CD14 ++ CD16 − ), intermediate (CD14 ++ CD16 + ) and non-classical (CD14 + CD16 ++ ) monocytes and circulating CD34 + CD45 dim progenitor cells were higher post-exercise in SPRINT, but unchanged in CONTEX. All post-exercise increases in SPRINT were back to pre-exercise levels at 2 h and 24 h. Conclusion: Acute exercise transiently increases circulating T ANG , TEM and progenitor cells with greater increases evident following very high intensity sprint exercise than following prolonged continuous paced endurance exercise.
KW - Angiogenic T cells
KW - Endothelial progenitor cells
KW - High intensity exercise
KW - Tie2 expressing monocytes
UR - http://www.scopus.com/inward/record.url?scp=85060599540&partnerID=8YFLogxK
U2 - 10.1007/s00421-018-04065-7
DO - 10.1007/s00421-018-04065-7
M3 - Article
C2 - 30673849
AN - SCOPUS:85060599540
SN - 1439-6319
VL - 119
SP - 743
EP - 752
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
IS - 3
ER -
