TY - JOUR
T1 - Determination of microbial numbers in anaerobically digested biofertilisers
AU - Coelho, Janerson Jose
AU - Prieto, Maria Luz
AU - Hennessy, Aoife
AU - Casey, Imelda
AU - Woodcock, Tony
AU - Kennedy, Nabla
N1 - Funding Information:
This work was supported by Conselho Nacional de Desenvolvi-mento Científico e Tecnológico (CNPq) – Brazil, [grant number 232596/2014-0].
Funding Information:
This work was supported by Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq)?Brazil, [grant number 232596/2014-0]. The authors gratefully acknowledge all biogas plant operators for contributing samples of digestate, and Angela Bywater of the Anaerobic Digestion Network (ADNet) for expert advice.
Publisher Copyright:
© 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - This study aimed to quantity total numbers of bacteria, fungi and archaea in different types of commercial liquid anaerobic digestates, and to identify common patterns in their microbial numbers post-digestion and possible implications of their use as biofertiliser. Relationships between microbial numbers and physical–chemical traits of the digestates were also investigated. Quantification was performed using culturable and molecular (quantitative PCR) approaches. Bacterial and fungal CFUs ranged up to five orders of magnitude (105–1010; 0–105 g−1 DW, respectively) between different types of anaerobic digestates. Bacterial, archaeal and fungal gene copy numbers (GCN) varied by two orders of magnitude (108–1010; 107–109; 104–106 g−1 DW, respectively) between digestates. All microbial variables analysed showed significant differences between the different types of anaerobic digestate investigated (p < 0.05). Culturable microbial numbers for fungi (6.43 × 104 CFU g−1 DW) were much lower than for bacteria (2.23 × 109 CFU g−1 DW). Gene copy numbers were highest for bacteria (16S) (1.09 × 1010 g−1 DW), followed by archaea (16S) (5.87 × 108 g−1 DW), and fungi (18S) (1.77 × 106 g−1 DW). Liquid anaerobic digestates were predominantly dominated by bacteria, followed by archaeal and fungal populations. At 50% similarity level, the microbial profiles of the eleven anaerobic digestates tested separated into just two groups, indicating a broad relative degree of similarity in terms of microbial numbers. Higher bacterial (16S) GCN was associated with low OM and C/N ratio in digestates.
AB - This study aimed to quantity total numbers of bacteria, fungi and archaea in different types of commercial liquid anaerobic digestates, and to identify common patterns in their microbial numbers post-digestion and possible implications of their use as biofertiliser. Relationships between microbial numbers and physical–chemical traits of the digestates were also investigated. Quantification was performed using culturable and molecular (quantitative PCR) approaches. Bacterial and fungal CFUs ranged up to five orders of magnitude (105–1010; 0–105 g−1 DW, respectively) between different types of anaerobic digestates. Bacterial, archaeal and fungal gene copy numbers (GCN) varied by two orders of magnitude (108–1010; 107–109; 104–106 g−1 DW, respectively) between digestates. All microbial variables analysed showed significant differences between the different types of anaerobic digestate investigated (p < 0.05). Culturable microbial numbers for fungi (6.43 × 104 CFU g−1 DW) were much lower than for bacteria (2.23 × 109 CFU g−1 DW). Gene copy numbers were highest for bacteria (16S) (1.09 × 1010 g−1 DW), followed by archaea (16S) (5.87 × 108 g−1 DW), and fungi (18S) (1.77 × 106 g−1 DW). Liquid anaerobic digestates were predominantly dominated by bacteria, followed by archaeal and fungal populations. At 50% similarity level, the microbial profiles of the eleven anaerobic digestates tested separated into just two groups, indicating a broad relative degree of similarity in terms of microbial numbers. Higher bacterial (16S) GCN was associated with low OM and C/N ratio in digestates.
KW - Anaerobic digestates
KW - archaea
KW - bacteria
KW - fungi
KW - qPCR
UR - http://www.scopus.com/inward/record.url?scp=85101457104&partnerID=8YFLogxK
U2 - 10.1080/09593330.2019.1645214
DO - 10.1080/09593330.2019.1645214
M3 - Article
C2 - 31314692
AN - SCOPUS:85101457104
SN - 0959-3330
VL - 42
SP - 753
EP - 763
JO - Environmental Technology (United Kingdom)
JF - Environmental Technology (United Kingdom)
IS - 5
ER -