TY - JOUR
T1 - Effect of seaweed supplementation on tocopherol concentrations in bovine milk using dispersive liquid-liquid microextraction
AU - Quigley, Andrew
AU - Walsh, Siobhán W.
AU - Hayes, Eva
AU - Connolly, Damian
AU - Cummins, Wayne
N1 - Funding Information:
The authors would like to thank the Irish Research Council [grant number GOIPG/2015/3681 ] for financial support of this work.
Publisher Copyright:
© 2018
PY - 2018/8/15
Y1 - 2018/8/15
N2 - A dispersive liquid-liquid microextraction (DLLME) method, combined with HPLC-UV detection, was developed for the extraction and preconcentration of δ-tocopherol from bovine milk. This method was used to study the effect of supplementing cow feed with the seaweed Ascophyllum nodosum on vitamin content in milk. The optimal experimental conditions were determined: 200 μL of chloroform (extraction solvent), 1.0 mL of ethanol (dispersive solvent), 5 mL of water (aqueous phase). Under these optimal conditions the DLLME method provided linearity in the range 0.01 μg/mL to 8 μg/mL with R2 values of 0.998. Limit of detection (LOD) was 0.01 μg/mL, while the enrichment factor was 89. Cow feed that was supplemented with Ascophyllum nodosum was shown to increase δ-tocopherol levels from 3.82 μg/mL to 5.96 μg/mL.
AB - A dispersive liquid-liquid microextraction (DLLME) method, combined with HPLC-UV detection, was developed for the extraction and preconcentration of δ-tocopherol from bovine milk. This method was used to study the effect of supplementing cow feed with the seaweed Ascophyllum nodosum on vitamin content in milk. The optimal experimental conditions were determined: 200 μL of chloroform (extraction solvent), 1.0 mL of ethanol (dispersive solvent), 5 mL of water (aqueous phase). Under these optimal conditions the DLLME method provided linearity in the range 0.01 μg/mL to 8 μg/mL with R2 values of 0.998. Limit of detection (LOD) was 0.01 μg/mL, while the enrichment factor was 89. Cow feed that was supplemented with Ascophyllum nodosum was shown to increase δ-tocopherol levels from 3.82 μg/mL to 5.96 μg/mL.
UR - http://www.scopus.com/inward/record.url?scp=85048579392&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2018.06.013
DO - 10.1016/j.jchromb.2018.06.013
M3 - Article
C2 - 29902754
AN - SCOPUS:85048579392
SN - 1570-0232
VL - 1092
SP - 152
EP - 157
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -